Abstract:
Abstract— Garcinia quaesita Pierre has growing demand presently
in the world for its remarkable medicinal value and has tremendous
potential for introduction into home gardens as a cash crop. This
experiment was designed to establish a protocol for in vitro
propagation of Garcinia to fulfill the high demand of good quality
planting material with the objectives of finding suitable sterilization
and regeneration media.
In experiment one, three treatments were tested to identify the
best sterilization procedure using 1-3 cm long shoot tips cultured on
Woody Plant Medium (WPM). As treatments, 0.1% Captan (1 hour)
+ 30% Clorox (20 minutes), 0.1% Captan (1 hour) + 30% Clorox
(20 minutes) + 50% ethanol (5 minutes) and 0.1% Captan (1 hour) +
40% Clorox (20 minutes) + 50% ethanol (5 minutes) were used.
Among those, treatment three showed the best performance (26.66%
sterile culture, 2.22% bacterial contamination, 2.22% dead culture
and 68.8% fungal contamination) compared to other treatments after
4 weeks. Therefore, treatment three was used for further experiments.
In experiment two, apical buds were cultured on WPM
supplemented with three concentrations of BAP at 3.0, 5.0, 10.0 mgL-
1 alone and 3.0 mgL-1 BAP with 0.5 mgL-1 NAA. The highest shoot
length (0.68 cm), the highest numbers of leaves (1.55 ± 1.01) and the
highest sterile culture percentage (44.55%) were observed in medium
containing 3.0 mgL-1 BAP with 0.5mgL-1 NAA after 4 weeks
(p=0.7089). There were not significant difference of shoot height
among treatments. Leaf explant of Garcinia were cultured on WPM
containing four levels of BAP (1, 2, 4, 8 mgL-1) with 0.5 mgL-1 NAA.
Callus development from the leaf explants couldn’t observed during
the time period and some were contaminated after 7 weeks.
WPM containing 3.0 mgL-1 BAP with 0.5 mgL-1 NAA was the best
treatment for in vitro propagation of Garcinia.
