Tissue Culture Protocol for Production of Planting Materials of Apple by Using Shoot Tip Culture

Abstract

Abstract—Shoot tip explants were taken from in vitro germinated apple seedlings using imported apple (Red Delicious). Apple seeds were germinated in hormone free Murashige and Skoog (MS) medium. In vitro raised shoot tips were transferred to different concentrations (0.0, 0.5, 1.0 1.5, 2.0, 2.5, 3.0, 3.5, 4, 4.5, 5 mgL-1) of Benzyl Amino Purine (BAP) in MS medium for shoot proliferation. The highest rate of shoot multiplication was determined by number of uniform sized shoots per propagule. Significantly highest mean number of shoots was recorded in 4 mgL-1BAP in MS medium. Subsequently, shoots were transferred into rooting media. Five replicates consisting two levels as full and half strength MS media and six levels of Indole Butric Acid (IBA) concentrations (0.0, 1.0, 2.0, 3.0, 4.0, 5.0 mgL-1) were tested. Significantly highest mean number of roots was observed in half strength MS medium with 2 mgL-1 IBA for root induction