Abstract:
Abstract—Shoot tip explants were taken from in vitro germinated
apple seedlings using imported apple (Red Delicious). Apple seeds
were germinated in hormone free Murashige and Skoog (MS)
medium. In vitro raised shoot tips were transferred to different
concentrations (0.0, 0.5, 1.0 1.5, 2.0, 2.5, 3.0, 3.5, 4, 4.5, 5 mgL-1) of
Benzyl Amino Purine (BAP) in MS medium for shoot proliferation.
The highest rate of shoot multiplication was determined by number of
uniform sized shoots per propagule. Significantly highest mean
number of shoots was recorded in 4 mgL-1BAP in MS medium.
Subsequently, shoots were transferred into rooting media. Five
replicates consisting two levels as full and half strength MS media
and six levels of Indole Butric Acid (IBA) concentrations (0.0, 1.0,
2.0, 3.0, 4.0, 5.0 mgL-1) were tested. Significantly highest mean
number of roots was observed in half strength MS medium with 2
mgL-1 IBA for root induction
